aseA [2018-02-09T00:34:01.000Z]

As(III) efflux pump

locus: BSU05340

pI: 8.97

mw: 47.16 kDa

protein length: 435 aa

gene length: 1305 bp

function: resistance to arsenite

product: As(III) efflux pump

essential: no

synonyms: ydfA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.11|Resistance against toxic metals]

Gene

Coordinates: 579,889 → 581,196

The protein

Paralogous protein(s)

• [protein|EB8E3F88BC1ED2BAD5A98D609EDAA002261150A5|YwrK]

Expression and Regulation

Operons

• genes: [gene|D169C58739F17EE0D341D015592FF07CBBE1B6E1|aseR]-[gene|C0E921ECAEE12DEC32A5DEE866762D6971360FA1|aseA]-[gene|6BA4A8575050B1BA45FF98E1F3B1F27F6C24A5E4|ydzS/1]-[gene|009BB4460A9374A8259DE7BD05724467198E0A90|ydzS/2]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|D169C58739F17EE0D341D015592FF07CBBE1B6E1|AseR]: repression, [Pubmed|15948947], in [regulon|D169C58739F17EE0D341D015592FF07CBBE1B6E1|AseR regulon]

  regulation

  • induced by As(III) ([protein|D169C58739F17EE0D341D015592FF07CBBE1B6E1|AseR]) [Pubmed|15948947]

Biological materials

Mutant

• MGNA-C141 (ydfA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2139 NBRP B. subtilis, Japan]
• BKE05340 (Δ[gene|C0E921ECAEE12DEC32A5DEE866762D6971360FA1|aseA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGATGTCAATCTAATGC, downstream forward: _UP4_TAAAAGGACAAGGTCACTAT
• BKK05340 (Δ[gene|C0E921ECAEE12DEC32A5DEE866762D6971360FA1|aseA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGATGTCAATCTAATGC, downstream forward: _UP4_TAAAAGGACAAGGTCACTAT

References

• 15948947